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SouthernBiotech col1a1
Col1a1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+type+i+collagen+antibody/Goat+Anti-Type+I+Collagen-UNLB/pm42806145-1039-0-2
Average 97 stars, based on 466 article reviews
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Related Articles

Incubation:

Article Title: High-resolution in vivo kinematic tracking with customized injectable fluorescent nanoparticles
Article Snippet: Then, the sections were blocked with 10% horse serum (Thermo Fisher Scientific, #16050114) in 0.1 M PB solution containing 0.3% Triton X (PBTx) for 30 min at room temperature. .. Sections were then incubated in PBTx solution containing 1% horse serum and goat anti–type I collagen antibody (1:500; SouthernBiotech, #1310-01) overnight at room temperature. .. Sections were then washed four times with 0.1 M PB solution and further incubated in PBTx solution containing donkey anti-goat antibody conjugated to CF488A (Sigma-Aldrich, #SAB4600032) for 1.5 hours at room temperature.

Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension.
Article Snippet: .. Membranes were incubated overnight at 4°C with anti‐fibronectin antibody (1:1000; Sigma‐ Aldrich, F3648), anti‐Type I collagen antibody (1:1000; Southern Biotech, 1310‐01), anti‐α‐SMA antibody (1:500, Santa Cruz Biotechnology, SC‐32251), and anti‐GAPDH (1:10,000; EMD Millipore, MAB374). .. The membranes were incubated with secondary anti‐rabbit (1:10,000; Invitrogen, A16035), anti‐goat (1:10,000; Invitrogen, A16017), or anti‐ mouse (1:10,000; Invitrogen, A16005) for 1 h at room temperature.

Western Blot:

Article Title: Pharmacological Inhibition of STAT6 Ameliorates Myeloid Fibroblast Activation and Alternative Macrophage Polarization in Renal Fibrosis
Article Snippet: Hematoxylin, VECTASTAIN ® Elite ABC-HRP Kit, Peroxidase (Rabbit IgG) PK-6101, Avidin/Biotin Blocking kit, Antigen unmasking solution and ImmPACT ® DAB Substrate, Peroxidase (HRP) (Vector Laboratories Burlingame, CA, USA). .. The primary and secondary antibodies used for the Western blot were GAPDH (monoclonal, 1:5000, EMD Millipore, MAB374, RRID : AB_2107445), anti-α-SMA antibody (monoclonal, 1:500, Santa Cruz, SC-32251, RRID: AB_262054), anti-type I collagen antibody (polyclonal, 1:1000, SouthernBiotech, 1310-01, RRID: AB_2753206), anti-fibronectin antibody (polyclonal,1:1000, Sigma-Aldrich, F3648, RRID : AB_476976), phospho-STAT6 (Tyr641) antibody (polyclonal, 1:500, cell Signaling Technology, 9361, RRID : AB_331595) and STAT6 Antibody (polyclonal, 1:500, Cell Signaling Technology, 9362, RRID: AB_2271211); and secondary anti-rabbit (polyclonal,1:10000, Thermo Fisher Scientific A16035, RRID: AB_2534709), anti-goat (polyclonal,1:10000, Thermo Fisher Scientific, A16005, RRID: AB_2534679), and anti-mouse (polyclonal,1:10000, Thermo Fisher Scientific, A16017, RRID: AB_2534691), respectively. .. The primary antibodies used in , for immunohistochemistry analysis were phospho-STAT6 (Tyr641) rabbit antibody (monoclonal, Thermo Fisher Scientific Cat# 700247, RRID: AB_2532305).

Immunohistochemical staining:

Article Title: Deletion of p38 MAPK in macrophages ameliorates peritoneal fibrosis and inflammation in peritoneal dialysis
Article Snippet: .. The primary antibodies used for immunohistochemical and immunofluorescence studies were as follows: anti-phospho-p38 MAPK antibody (#9211, Cell Signaling Technology, Boston, MA), anti-F4/80 antibody (MCA497GA, Bio-Rad, Hercules, CA), anti-MAC-2 antibody (CL8942F, Cedarlane, Ontario, Canada), and anti-α-smooth muscle actin (αSMA) antibody (ab5694, Abcam, Cambridge, UK), and anti-type I collagen antibody (1310-01, Southern Biotechnology Associates, Alabama, UK). .. The type I collagen antibodies were diluted at 1:50, while the other antibodies were used at a 1:100 dilution. pCDNA3 Flag p38 alpha was a gift from Roger Davis (Addgene plasmid # 20351; http://n2t.net/addgene:20351 ; RRID:Addgene_20351) .

Immunofluorescence:

Article Title: Deletion of p38 MAPK in macrophages ameliorates peritoneal fibrosis and inflammation in peritoneal dialysis
Article Snippet: .. The primary antibodies used for immunohistochemical and immunofluorescence studies were as follows: anti-phospho-p38 MAPK antibody (#9211, Cell Signaling Technology, Boston, MA), anti-F4/80 antibody (MCA497GA, Bio-Rad, Hercules, CA), anti-MAC-2 antibody (CL8942F, Cedarlane, Ontario, Canada), and anti-α-smooth muscle actin (αSMA) antibody (ab5694, Abcam, Cambridge, UK), and anti-type I collagen antibody (1310-01, Southern Biotechnology Associates, Alabama, UK). .. The type I collagen antibodies were diluted at 1:50, while the other antibodies were used at a 1:100 dilution. pCDNA3 Flag p38 alpha was a gift from Roger Davis (Addgene plasmid # 20351; http://n2t.net/addgene:20351 ; RRID:Addgene_20351) .

Article Title: Pharmacological Inhibition of STAT6 Ameliorates Myeloid Fibroblast Activation and Alternative Macrophage Polarization in Renal Fibrosis
Article Snippet: The primary antibody used in , for immunofluorescence analysis was mouse anti-α-SMA (monoclonal, 1:400, Santa Cruz, SC-32251, RRID: AB_262054); the secondary antibody Alexa Fluor 488-conjugated donkey anti-mouse antibody (polyclonal,1:400, Molecular Probes, A-21202, RRID: AB_141607). .. The primary antibodies used in , for immunofluorescence analysis anti-fibronectin antibody (polyclonal,1:400, Sigma-Aldrich, F3648, RRID: AB_476976), and anti-type I collagen antibody (polyclonal, 1:400, SouthernBiotech, 1310-01, RRID: AB_2753206); the secondary antibodies Alexa Fluor 488 donkey anti-rabbit IgG (polyclonal, 1:400, Thermo Fisher Scientific, A-21206, RRID: AB_2535792) and Alexa Fluor 488-conjugated donkey anti-goat antibody (polyclonal, 1: 400, Thermo Fisher Scientific, A-11055, RRID: AB_2534102). .. Wild-type (WT) C57BL/6 mice were purchased from the Jackson Laboratory.

Cell-Signaling:

Article Title: Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis
Article Snippet: LPC (Sigma, St. Louis, MO) was dissolved as previously described ( ). .. Primary antisera employed for the studies include anti-alpha smooth muscle actin (αSMA) (ab124964) and anti-fibronectin (ab2413) antibodies from Abcam (Cambridge, MA), anti-GAPDH (MAB374) from Millipore Sigma, anti-β-actin (sc-47778) from Santa Cruz Biotechnologies (Santa Cruz, CA) and anti-Lyve1 (AF2125) from R&D systems (Minneapolis, MN), anti-F4/80 (70076) and anti-phospho Yap (Ser127) (4911) from Cell Signaling Technology (Danvers, MA), anti-Yap1 (sc-101199) from Santa Cruz Biotechnologies (Santa Cruz, CA), anti-type I collagen antibody (1310-01) from Southern Biotech. .. Recombinant human VCAM1 (rhVCAM1) (862-VC) was obtained from R&D systems.

Blocking Assay:

Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension.
Article Snippet: Sections were incubated with BlockAidTM blocking solution (Invitrogen; B10710) to block nonspecific binding. .. After blocking, the sections were stained with anti‐fibronectin antibody (1:400; Sigma‐Aldrich, F3648) followed by Alexa Fluor 488‐conjugated donkey anti‐rabbit antibody (1:400, Invitrogen, A21206), anti‐Type I collagen antibody (1:400; SouthernBiotech, 1310‐01) followed by Alexa Fluor 488‐conjugated donkey anti‐goat antibody (1:400; Invitrogen, A11055), or anti‐α‐SMA antibody (1:400; Santa Cruz Biotechnology, SC‐32251) followed by Alexa Fluor 488‐conjugated donkey anti‐mouse antibody (Invitrogen; A21202) (An et al., 2020). ..

Staining:

Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension.
Article Snippet: Sections were incubated with BlockAidTM blocking solution (Invitrogen; B10710) to block nonspecific binding. .. After blocking, the sections were stained with anti‐fibronectin antibody (1:400; Sigma‐Aldrich, F3648) followed by Alexa Fluor 488‐conjugated donkey anti‐rabbit antibody (1:400, Invitrogen, A21206), anti‐Type I collagen antibody (1:400; SouthernBiotech, 1310‐01) followed by Alexa Fluor 488‐conjugated donkey anti‐goat antibody (1:400; Invitrogen, A11055), or anti‐α‐SMA antibody (1:400; Santa Cruz Biotechnology, SC‐32251) followed by Alexa Fluor 488‐conjugated donkey anti‐mouse antibody (Invitrogen; A21202) (An et al., 2020). ..

other:

Article Title: Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis.
Article Snippet: LPC (Sigma, St. Louis, MO) was dissolved as previously described (8).



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Image Search Results


AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Journal: Cardiovascular Therapeutics

Article Title: CircIdh2 Attenuates Endothelial‐to‐Mesenchymal Transition and Myocardial Fibrosis and Exhibits Translational Potential

doi: 10.1155/cdr/2005876

Figure Lengend Snippet: AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies against Collagen I (1:200, #66761‐1‐Ig, Proteintech) and TGF‐ β 1 (1:200, #81746‐2‐RR, Proteintech).

Techniques: Marker, In Vivo, H&E Stain, Staining, Western Blot, Analysis