col1a1 (SouthernBiotech)
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Col1a1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+type+i+collagen+antibody/Goat+Anti-Type+I+Collagen-UNLB/pm42806145-1039-0-2
Average 97 stars, based on 466 article reviews
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Incubation:Article Title: High-resolution in vivo kinematic tracking with customized injectable fluorescent nanoparticles Article Snippet: Then, the sections were blocked with 10% horse serum (Thermo Fisher Scientific, #16050114) in 0.1 M PB solution containing 0.3% Triton X (PBTx) for 30 min at room temperature. .. Sections were then incubated in PBTx solution containing 1% horse serum and goat Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension. Article Snippet: .. Membranes were incubated overnight at 4°C with anti‐fibronectin antibody (1:1000; Sigma‐ Aldrich, F3648), Western Blot:Article Title: Pharmacological Inhibition of STAT6 Ameliorates Myeloid Fibroblast Activation and Alternative Macrophage Polarization in Renal Fibrosis Article Snippet: Hematoxylin, VECTASTAIN ® Elite ABC-HRP Kit, Peroxidase (Rabbit IgG) PK-6101, Avidin/Biotin Blocking kit, Antigen unmasking solution and ImmPACT ® DAB Substrate, Peroxidase (HRP) (Vector Laboratories Burlingame, CA, USA). .. The primary and secondary antibodies used for the Western blot were GAPDH (monoclonal, 1:5000, EMD Millipore, MAB374, RRID : AB_2107445), anti-α-SMA antibody (monoclonal, 1:500, Santa Cruz, SC-32251, RRID: AB_262054), Immunohistochemical staining:Article Title: Deletion of p38 MAPK in macrophages ameliorates peritoneal fibrosis and inflammation in peritoneal dialysis Article Snippet: .. The primary antibodies used for immunohistochemical and immunofluorescence studies were as follows: anti-phospho-p38 MAPK antibody (#9211, Cell Signaling Technology, Boston, MA), anti-F4/80 antibody (MCA497GA, Bio-Rad, Hercules, CA), anti-MAC-2 antibody (CL8942F, Cedarlane, Ontario, Canada), and anti-α-smooth muscle actin (αSMA) antibody (ab5694, Abcam, Cambridge, UK), and Immunofluorescence:Article Title: Deletion of p38 MAPK in macrophages ameliorates peritoneal fibrosis and inflammation in peritoneal dialysis Article Snippet: .. The primary antibodies used for immunohistochemical and immunofluorescence studies were as follows: anti-phospho-p38 MAPK antibody (#9211, Cell Signaling Technology, Boston, MA), anti-F4/80 antibody (MCA497GA, Bio-Rad, Hercules, CA), anti-MAC-2 antibody (CL8942F, Cedarlane, Ontario, Canada), and anti-α-smooth muscle actin (αSMA) antibody (ab5694, Abcam, Cambridge, UK), and Article Title: Pharmacological Inhibition of STAT6 Ameliorates Myeloid Fibroblast Activation and Alternative Macrophage Polarization in Renal Fibrosis Article Snippet: The primary antibody used in , for immunofluorescence analysis was mouse anti-α-SMA (monoclonal, 1:400, Santa Cruz, SC-32251, RRID: AB_262054); the secondary antibody Alexa Fluor 488-conjugated donkey anti-mouse antibody (polyclonal,1:400, Molecular Probes, A-21202, RRID: AB_141607). .. The primary antibodies used in , for immunofluorescence analysis anti-fibronectin antibody (polyclonal,1:400, Sigma-Aldrich, F3648, RRID: AB_476976), and Cell-Signaling:Article Title: Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis Article Snippet: LPC (Sigma, St. Louis, MO) was dissolved as previously described ( ). .. Primary antisera employed for the studies include anti-alpha smooth muscle actin (αSMA) (ab124964) and anti-fibronectin (ab2413) antibodies from Abcam (Cambridge, MA), anti-GAPDH (MAB374) from Millipore Sigma, anti-β-actin (sc-47778) from Santa Cruz Biotechnologies (Santa Cruz, CA) and anti-Lyve1 (AF2125) from R&D systems (Minneapolis, MN), anti-F4/80 (70076) and anti-phospho Yap (Ser127) (4911) from Cell Signaling Technology (Danvers, MA), anti-Yap1 (sc-101199) from Santa Cruz Biotechnologies (Santa Cruz, CA), Blocking Assay:Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension. Article Snippet: Sections were incubated with BlockAidTM blocking solution (Invitrogen; B10710) to block nonspecific binding. .. After blocking, the sections were stained with anti‐fibronectin antibody (1:400; Sigma‐Aldrich, F3648) followed by Alexa Fluor 488‐conjugated donkey anti‐rabbit antibody (1:400, Invitrogen, A21206), Staining:Article Title: Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension. Article Snippet: Sections were incubated with BlockAidTM blocking solution (Invitrogen; B10710) to block nonspecific binding. .. After blocking, the sections were stained with anti‐fibronectin antibody (1:400; Sigma‐Aldrich, F3648) followed by Alexa Fluor 488‐conjugated donkey anti‐rabbit antibody (1:400, Invitrogen, A21206), other:Article Title: Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis. Article Snippet: LPC (Sigma, St. Louis, MO) was dissolved as previously described (8). |
